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In another study, patients with highly active colitis treated with infliximab in vitro were analyzed and a reduced T cell activation and proliferation was found [12].
The proliferation and cytokine production of draining lymph node cells in vitro were analyzed.
Growth curve and growth fold change data in vitro were analyzed similarly.
B6.SJL mice were given 5 × 10 OT-II cells and the proliferative responses of these donor cells in vitro were analyzed one to eight weeks later.
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The rat DC were characterized by flow cytometric analyses, and their ability to stimulate T cells in vitro was analyzed by mixed leukocyte reaction (MLR) assay.
Next, the invasivity of cells in vitro was analyzed to determine the effect of PKCα variants on the capacity of cells to invade 3D collagen.
Next, the invasivity of cells in vitro was analyzed to determine the effect of PKCα on the capacity of cells to invade 3D collagen.
Expression of recombinant plasmid (pVAX-CDPK1) in vitro was analyzed by IFA at 48 h post-transfection, and specific green fluorescence was observed in Marc-145 cells transfected with pVAX-CDPK1, whereas no fluorescence was observed in the cells transfected with pVAX I (Additional file 1: Figure S1).
Data obtained in vitro and in vivo were analyzed with Clampfit (Molecular Devices) and Matlab (Mathworks), respectively.
The in vitro data were analyzed by one-way ANOVA using SPSS-17 software, and the post hoc test was performed for multiple comparisons.
Differences of in vitro measurements were analyzed by in one-way ANOVA procedure (SAS Inc., Cary, NC, USA) version SPSS 19.0 (SPSS Inc., Chicago, IL, USA) to test the differences among treatments.
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