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The purified fusion protein produced in vitro was injected intravenously into juvenile pigs for pharmacokinetic study.
Purified BChE/hSA fusion protein produced in vitro was injected i.v. into 2 juvenile pigs of ~20 kg (10 mg/kg, using a converting equation of 720 units = 1 mg purified plasma huBChE [ 29]).
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Resulting bioluminescent cell lines (MdaA-.luc, MdaP.luc, MdaA+.luc) with bioluminescence in vitro were injected into the mammary fat pad of SCID mice and monitored for tumor growth.
The importance of this mechanism of anti-cancer activity was definitely proven in experiments where tumour cells resistant to trabectedin in vitro were injected into mice.
To evaluate whether HM1.24-ETA′ was also active in vivo, 2.5 × 10 INA-6 cells that demonstrated the lowest sensitivity to HM1.24-ETA′ treatment in vitro were injected intraperitoneally into 7-week-old SCID beige mice.
This form, labeled in vitro with 109Cd, was injected intravenously in mice, and the distribution of 109Cd was studied.
In vitro transcribed cRNA was injected together with the cRNA of the G protein-coupled inwardly-rectifying potassium channel GIRK, and the oocytes were analyzed by whole cell clamp analysis [ 11, 12].
In vitro transcribed cRNA was injected together with the cRNA of a G-protein coupled inwardly rectifying potassium channel, and the oocytes were analyzed by whole cell clamp as described [ 11, 12].
In the first method, in vitro transcribed SB11 mRNA was injected into 1-cell embryos from Tg(T2/OncZ, ß-actin:RFP) fish (Fig. 3 A).
600 pg of the in vitro transcribed eFLP mRNA was injected into the yolks of 1-cell stage zebrafish embryos in 3 nL volume.
For reversion of gene trap mutations, 25 75 pg of in vitro transcribed Cre mRNA was injected into 1-cell zebrafish embryos.
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