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The creation of cell lines became technologically feasible in the 1950s, when the first cell line that multiplied in vitro was generated using the cells of a cancer patient called Henrietta Lacks, also known as HeLa [ 3].
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Up-states in-vivo and in-vitro are generated through local recurrent synaptic excitation that is balanced and controlled by the activity of GABAergic interneurons [12], [21], [52], [53].
The template for in vitro translation was generated according to the sequence of pSGR-Luc-JFH1 (Cao et al., 2014) between the EcoRI and PmeI sites.
A recombinant biotinylated boC3d protein (for use in in vitro studies) was generated by endogenous biotinylation in Escherichia coli by means of the BirA holoenzyme synthetase.
In vitro oxLDL was generated by modification of low density cholesterol with malondialdehyde as well as cupper.
In vitro RNA was generated from these plasmids using T7 or T3 RNA polymerase, depending on the orientation of the fragment, and was treated with DNase to remove the plasmid.
To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time.
A Cas9 expression vector for SP6 in vitro transcription, pCS2+hSpCas9, was generated in this study.
In vitro PD model was generated by exposing SH-SY5Y cells to neurotoxin 6-hydroxydopamine (6-OHDA).
Here, an in vitro Wolbachia infection was generated in the Drosophila S2 cell line, and transcription profiles of infected and uninfected cells were compared by microarray.
A DNA template suitable for runoff in vitro transcription of hTR 353−451) was generated by restriction endonuclease digestion of plasmid pUC18-hTR 353−451) pUC18-hTR 353−451
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