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pri-miRNA-376a2, which was transcribed in vitro, was added to HeLa extracts competent for Drosha cleavage.
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PBMCs, either freshly separated or restimulated in vitro, were added at E T ratios of 10 1 and 50 1 onto autologous and heterologous fibroblasts.
Patients enrolled in this present study had an average serum drug level of 32 ± 14 mg/L, much lower than that of severely intoxicated patients (61 ± 25 mg/L in our previous series) [ 7]. in vitro, metformin was added to plasma to obtain an initial (toxic) concentration of 166 mg/L, or higher.
However, it remains unclear how it works in vitro as quercetin was added after LPS stimulation (signal 1).
In vitro, R136K-CBD was added to canine jugular vein EC and canine carotid artery smooth muscle cell (SMC) cultures to evaluate mitogenic response.
50 μL of antigen (supernatant of in vitro pyrogen assay) was added per well and incubated at room temperature for 2 h.
In vitro translated RHOBTB3 was added to the mixture containing bacterially expressed GST-PHD2 and anti-MYC-conjugated resin-bound MYC-tagged VHL.
To elucidate the effects of exogenous IL-32α on TNFα production in vitro, rIL-32α was added to RAW 264.7 cells in culture.
For in vitro assays, CLINME was added to the [I]-CLINME to achieve a specific activity of 3.7 GBq/ μmol and reconstituted in ethanol.
Selenium does not appear to act by simply modifying intake, absorption, excretion, or distribution of methylmercury, and direct effects of both selenium and vitamin E have been observed in vitro when methylmercury was added to cultured nervous tissue cells.
Forty-eight hours after transfection, 5-Ethynyl-2′-deoxyuridine (EdU) (50 μM) (Cell-LightTM EdU Apollo567 In Vitro Kit, RiboBio) was added and the cells were cultured for an additional 2 h.
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