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While other studies have examined extinct coding DNA function in vitro, this is the first example of the restoration of extinct non-coding DNA and examination of its function in vivo.
In vitro this is observed as de-differentiation in response to TGF-β2.
While a slow reaction occurs in vitro, this is likely not relevant to the natural reaction.
Although a link between SMAD activation and dendrite growth has been reported in vitro, this is not an invariable link.
In vitro this is assessed by the ability of cytokine-activated NK cells to induce lysis of the NK cell resistant Daudi cell line.
In vitro this is detected as an inability of OVA-stimulated splenocytes to produce IL-4 while producing significantly elevated amounts of IFNγ [ 3].
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Developed formulations were found to have prolonged drug release for up to 3 weeks in vitro; this was best fitted by the Higuchi model.
They were also evaluated for their haemolytic activity and did not show hydrolysis of sheep blood in vitro, this being another desirable phenotype in terms of lack of infectivity and pathogenicity.
Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis.
Eighty-five per cent of cultures established from oestrogen receptor (ER -positive tumours ER -positive in vitumoursis was functional in 66% of culturexpressedugh ER-posinivitroenothis was gradually lost over time.
Although increased chondrocyte mRNA for S100a8 and S100a9 could be induced by IL-1 in vitro, this was associated with an increase in cell and cartilage matrix staining for the two proteins.
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