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In vitro, stem cells can readily be engineered by inserting specifically tailored transgenes with antitumour effects to create tumour-seeking therapeutic vehicles.
The CRA assay is a non-clonogenic assay capable to reveal in vitro stem cells endowed with marrow-repopulating ability in vivo [11].
Due to an intense discussion about the use of hiPSCs in cellular therapies, since they are not completely safe, a lot of works, trying to establish in vitro stem cells derived from a variety of sources, has emerged.
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In vitro stem cell viability under transfection condition was evaluated by MTT assay.
To compensate, a strategy of in vitro stem cell amplification has been attempted in different research laboratories.
Datasets describing cellular interaction networks need to be integrated into predictive models of in vitro stem cell development.
This chapter focuses on progress in the design and characterization of artificial matrices that attempt to recapitulate microenvironmental cues for in vitro stem cell culture and differentiation.
In conclusion, designing specific microenvironments for in vitro stem cell culture, such as those containing bioactive material, will facilitate the development of customized exosomes encapsulating a beneficial composition of stem cells for cell-free therapeutic applications.
There are, however, still many gaps in the knowledge of in vivo and in vitro stem cell biology, and in understanding the mechanisms of a number of diseases which will benefit from stem cell therapy.
How to surpass in vitro stem cell differentiation, reducing cell manipulation, and lead the in situ regeneration process after transplantation, remains to be unraveled in bone tissue engineering (bTE).
In the presence of tissue-inductive stimuli, in vitro stem cell culture on silk fibroin/hyaluronan scaffolds resulted in more efficient tissue formation when measured by glycosaminoglycan and type-I and type-III collagen gene expression, as compared to plain silk fibroin scaffolds.
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