Sentence examples for vitro processing from inspiring English sources

An analysis of 209 pri-miRNA sequences identified 47 which are highly edited in the human brain and in vitro processing reactions indicated that while many edited pri-miRNAs were refractory to processing by Drosha or Dicer, the editing of selected miRNAs did not alter, or enhanced processing [ 193].

In vitro processing of semisynthetic substrates unrelated to the prelacticin 481 peptide demonstrated the broad substrate tolerance of LctM.

This is usually achieved by in vitro processing with the help of an exogenously added protease that cleaves a site introduced between the fusion partners.

MBP-GrB fusion protein was cleaved during secretion by an endogenous furin-like proteolytic activity in vivo, liberating enzymatically active GrB without the need of subsequent in vitro processing.

To circumvent the need for in vitro processing, we included a synthetic furin recognition motif between the protein domains of MBP-GrB fusion proteins and investigated potential in vivo cleavage by endogenous furin-like proteases from yeast.

However, in two other assays, in vitro processing of either fusion proteins or oligopeptides, this peptide proved to be the worst TEVp substrate of all 20 permutationsions tested, except ENLYFQP for which no cleavage could be observed at all [18].

First, we assayed Tat-K efficacy to prevent Kidins220 in vitro calpain processing.

Similarly to what we observed for in vitro-processing of SUMO chains by recombinant Wss1, the isolated WT protein complex accumulated sumoylated species of intermediate size as a result of the proteolysis of HMW-SUMO.

Likewise, an in vitro recombinant Gag processing experiment was also incapable of recapitulating the inhibition of CA-SP1 processing by bevirimat.

MMP-10 is, in vitro, capable of processing laminin-5, which may be an additional mechanism by which the enzyme enables cellular migration (Krampert et al. 2004).

Similarly, mutation of Rat1 results in diminished association of several 3′ end processing factors with 3′ ends of genes, although in vitro 3′ end processing itself is not affected [120,122].