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In addition, unlike monolayer in vitro models, the cardiac microtissues are not attached to a solid surface.
Using C2C12 myoblast cells as in vitro models, the enhanced bioactivity of BMP-2 was attributed primarily to the stimulation from 6-O-sulfated chitosan (6SCS), while 2-N-sulfate was subsidiary group with less activation.
Using in vitro models, the mechanics as well as surgical techniques for mitral valves (MV) and MV devices can be studied in a more controlled environment with minimal monetary investment and risk.
Therefore, in human in vitro models, the relevance of endotoxin as a confounder could be less significant than in mice.
Through the use of in vitro models, the biology of the cells involved in bone remodelling has made important advances.
Our approach combines the use of two in vitro models, the 1C11 cell line and neurosphere cultures.
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We have recently tested an in vitro model of esophageal inflammation.105 In this in vitro model the mucosa/submucosa is freed by removing the circular and longitudinal muscle, and by sharp dissection at the level of the submucosa, and segments of the mucosal tube are tied at both ends, forming a closed sac (Figure 12).
We tested whether this might be a direct effect of CT by using an in vitro model, the growth plate from the fetal pig scapula.
In our in vitro model, the basal 1 h uptake of PSMA-binding peptide differed between castration-resistant cells and the androgen-sensitive cell line.
From the in vitro model, the optimal dosage form of a drug can be designed and verified in vivo.
This observation suggests that in this in vitro model, the MBNL1 cell localization is unaffected by the expression of the pathological CUG expansions.
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