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In i n vitro models, however, the active synthesis of new matrix occurs independently of biomechanical loading.
These data are based only on in vitro models, however, and correlation with the in vivo situation is not always a straightforward matter.
The endothelial cell monolayer is one of the most commonly used in vitro models; however, it only represents a simplified view of the BBB.
A major disadvantage of these in vitro models, however, is that the native tissue environment is lost during cell isolation, which might affect cell behaviour [ 15].
Prior to being introduced into large clinical trials, novel combination therapies should be supported by in vitro models, however, healthy skepticism should be maintained since robust preclinical data may not always translate into meaningful clinical benefit, as illustrated by the recent disappointing results of combination trials with an FTI or an mTOR inhibitor.
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In our in vitro model, however, TGFβ1 stimulation did not induce conversion to the myofibroblast phenotype [ 6].
However, these in vitro models are still useful to study amastigotes in simple controllable conditions.
Attempts to establish in vitro models were unsuccessful, however.
Thus, in vitro models are still required.
Beta-synuclein counteracts aggregation of alpha-synuclein in vitro and in animal models, however it is not clear whether this effect occurs in human Lewy body dementia (LBD) diseases.
However, in vitro models offer many advantages with low cost, easy sampling accessibility and no ethical issues.
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