Considering these promising results, despite the inherent simplifications of any in vitro model, further animal research, preferably using a whole animal model, seems appropriate.
This in vitro model further confirmed the docking results that WA might enter the HRas-RasGAP complex to interfere with the interaction of HRas-GTP and RasGAP.
In this paper, we review the current status of knowledge of the trabecular meshwork and how the current advances in tissue engineering techniques might be applied in an effort to engineer a synthetic trabecular meshwork as a 3D in vitro model to further advance glaucoma research.
Our preliminary data are qualitative and limited to an in vitro model, and further in-depth studies will be needed to fully clarify the pathogenetic mechanisms involved in vertical transmission and/or pregnancy interference in HHV-8-infected women.
To establish an in vitro model for further functional characterization of CapG in ovarian carcinomas, four ovarian carcinoma cell lines were analyzed.
Whether this result was due to properties of specific viral strains or a difference attributable to the in vitro model needs further clarification.
Future studies will include determining if addition of type-III and other collagens into our in vitro model system further modifies any of the disease-specific responses identified in this report.
Because hCMEC/D3 cells possess a H+/OC antiporter, like rodent BBB, and show similar mRNA expression of identified organic cation transporters to those in rat BBB model cells, hCMEC/D3 cells should be a good in vitro model for further studies on the H+/OC antiporter.
More recently, the human cholangiocyte H69 cell line was also shown to be susceptible to RV infection in a way that paralleled the murine model of BA [ 24], providing a human in vitro model to further study the pathogenic mechanisms involved in human BA.
Expression of invasion-associated markers and pathways (AKT, PI3 kinase, STAT1), identified in our in vitro models, will be further investigated in clinical tumor samples, with a focus on high grade, metastasizing and invasive cancers.
