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Partial inhibition of fluciclovine uptake by BCH and MeAIB can be demonstrated in vivo similar to previous in vitro modeling.
Analysis of the underlying transcriptional mechanisms will likely guide rational design of conditions to push iPS-CM further toward a more adult-like state for more predictive in vitro modeling.
The limitation of the static environment in Boyden devices has been overcome both by the use of parallel-plate flow chambers and by custom models mimicking the in vivo conditions, along with widespread microfluidic approaches to in vitro modeling.
By in vitro modeling increased mtDNA damage induced by rotenone, an inhibitor of mitochondrial complex I, in the RPE, we found that the phagocytic activity was not altered but that there were: 1) increased autophagic markers, 2) decreased lysosomal activity, 3) increased exocytotic activity and 4) release of chemoattractants.
Sufficient amounts of CSF is relatively hard to obtain for cell differentiation but the observed fact that extended cell culturing of neural stem cells is possible makes CSF an interesting option for fully human based disease-specific in vitro modeling.
Our experiments demonstrate two comparable approaches of in vitro modeling lipodystrophies with patient-specific iPSCs, and support a regulatory role of lamin A in the terminal differentiation stage of adipogenesis.
Similar(11)
"In vitro models are cleaner".
Central to achieving this is improved in vitro models.
The Bakerian Lecture, 1977: In Vitro Models for Photosynthesis, Proceedings of the Royal Society A Mathematicall, Physical and Engineering Sciences, 362 (1710) 281-303.
Dembele, L. et al. Towards an in vitro model of Plasmodium hypnozoites suitable for drug discovery.
Kahn, J. et al. Gene expression profiling in an in vitro model of angiogenesis.
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