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There is currently great progress in the development and standardization of stable in vitro liver cell culture techniques.
In vitro liver cell models also have various applications in toxicology: screening of cytotoxic and genotoxic compounds, evaluation of chemoprotective agents, and determination of characteristic liver lesions and associated biochemical mechanisms induced by toxic compounds.
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CYP1A1 was strongly induced in both in vivo and in vitro liver cells by TNT.
To further understand the gene function influenced by TNT exposure to in vivo and in vitro liver cells, we conducted a canonical pathway analysis using the Ingenuity pathway analysis tool.
An ideal three-dimensional (3D) in vitro liver parenchymal cell culture platform should restore cell cell and cell matrix interactions, as well as normal hepatocyte polarity.
The use of in vitro human liver cell models is an attractive approach in toxicogenomic studies designed to analyze gene expression changes induced by a toxic chemical.
We compared the gene expression profiles of in vitro primary liver cells with the gene expression profiles of in vivo liver tissue of rats exposed to TNT.
Here, we investigated the cytotoxicity of TiO2 NPs in vitro using four liver cell lines: human hepatocellular carcinoma cell line SMMC-77211), human liver cell line HL-77022), rat hepatocarcinoma cell line CBRH-79199) and rat liver cell line (BRL-3A).
The cells examined were primary cultures of epithelial liver cells, long-term cultures of epithelial liver cells, in vitro transformed epithelial liver cell lines and liver tumour-cell lines; mesenchymal cells from liver and skin were also examined.
This conclusion is supported by recent in vitro studies in mouse liver cell lines (22).
It has been observed that identification of liver toxicity can indeed be achieved by in vitro metabolomics using the HepG2 liver cell line.
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