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Tn 5 transposition, in vitro, is only ~1% efficient [ 10], so the kanamycin resistance gene (Kan R ) was incorporated between the two fluorescent protein cassettes.

The paired segments are generally shorter than those observed in group I introns consistent with the fact that the shortest form of DiGIR1 shown to catalyse branching in vitro is only 179 nt (Nielsen et al, 2005).

As the IC50 of STX140 in BCRP-expressing cells in vitro is only 1.5 2  μ M, this dose may be regularly or continuously exceeded in the in vivo model at a 20 mg kg−1 per day dose, resulting in good efficacy of STX140 in BCRP-expressing tumours in vivo.

Interestingly, FOXP3 DNA binding in vitro was only detected upon removal of the N-terminal region (FOXP3-ΔN); truncating the corresponding N-terminal region of Foxp1 also enhanced DNA binding.

However, changes to acute cocaine re-exposure in vitro were only detected in the frequency of sEPSCs, but not sIPSCs.

Maximal diffuse colony formation in vitro was only obtained in the simultaneous presence of adequate numbers of compact colonies.

As shown in Fig. 2A, parasite-specific IFN-γ responses in vitro are only detectable at day 70, when infection is waning.

However, at 48 HPI the mean fold difference of the gene expression in the liver compared to the bacteria in vitro was only of 1.07, a difference that was not statistically significant (p value of 0.83 as calculated by T-Test).

It is important to realize that in vitro susceptibility is only one of the factors to consider for drawing clinical inferences.

In vitro it is only possible to achieve relatively low rates of hepatoma cell infection by Plasmodium sporozoites, which makes biochemical analysis of the liver stage very difficult.

Finally, in vitro cytotoxicity is only one aspect of T-cell function and does not always correlate with effective T-cell responses in vivo [12].