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Atypical PrPres was found in their brains, proving that fibril-derived atypical PrPres generated in vitro is able to propagate in vivo (Fig. 3a).
Additionally, this behavioural effect is corroborated by the demonstration that HCN blockade on cold-sensing neurons in vitro is able to increase the threshold of cold detection, thereby directly lowering the excitability of this subclass of nociceptors.
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We also demonstrated that Brachyury is immunogenic and that Brachyury-specific CD8+ T cells expanded in vitro are able to lyse Brachyury-positive tumor cells.
We showed that ETM in vitro was able to stimulate TSC proliferation and better preserve the stemness of TSCs than plastic culture surfaces.
That is, minimally cultured individual colonies generated in vitro are able to generate tumors in vivo that resemble the parental adenocarcinomas from which they were obtained.
These results suggest that rat zona-free embryos cultured in vitro are able to develop to blastocyst stage; however, postimplantation development is incomplete.
Gonzalez-Juarrero et al reported that lung DCs pulsed with Ag85 (LDC-Ag85) in vitro were able to prime naive CD4+ T cells in vivo after adoptively transfer [25].
A persistent EGF supplementation in vitro was able only partially rescue the effect of Erk1/2 downregulation in c-Met depleted hepatocytes and restore to some extent the levels of cdc2, Aurora A and B, and Mad2.
Mature fibrocytes exposed to TGF-β in vitro are able to develop further into myofibrocytes, a myofibroblast-like population of cells that express α-SMA and are able to contract collagen gels, in an in vitro model of wound contraction [7].
In fact, in the mouse, two papers showed that haploid cells generated in vitro were able to fertilize oocytes.
Additionally, microvessel fragments isolated from multiple adult tissues in vitro are able to develop intact networks after implantation [ 27, 28].
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