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Using a mechanical compression device, the axon of dorsal root ganglion (DRG) neurons buckled in vitro into a profile that resembled the morphology of the RA-I receptor.
This is the first time that a viral genome is packaged in vitro into a membrane vesicle.
To perform screens of genes involved in the cortical branched actin pathway, genes were knocked down with esiRNAs (Sigma), which correspond to long dsRNAs processed in vitro into a collection of short siRNAs.
When cultured on dermal fibroblasts, the cord epithelial cells were able to differentiate in vitro into a stratified multilayered epithelium expressing molecules characteristic of keratinocyte differentiation after exposure to air, and maintaining the expression of keratins K18 and K19, typical of the umbilical cord epithelium.
The ESCs isolated from the inner cell mass of blastocyst-stage embryos are undifferentiated, pluripotent cells[27, 28], and can be induced to differentiate in vitro into a wide range of different cell types[29 36].
MSCs are a population of undifferentiated multipotent adult cells that naturally reside within the human body and are generally defined as plastic-adherent, fibroblast-like cells possessing extensive self-renewal properties and the potential to differentiate in vivo and in vitro into a variety of mesenchymal lineage cells [3].
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Accumulated evidence suggested that hPSC could develop in vitro into an extraembryonic lineage (trophoblast (TB)) that is typically inaccessible to pluripotent embryonic cells during embryogenesis.
P22 coat and scaffolding proteins assemble in vitro into an icosahedral procapsid, which then expands during DNA packaging (maturation).
This domain folds in vitro into an amphipathic poly-Proline II conformation [ 13] that in turn forms an expanded helix able to assemble both with itself [ 16] and with membrane lipids [ 17].
We have previously shown the potential of poly-lactic acid (PLA), an FDA approved polymer synthesized into a microfiber scaffold, to develop in vitro into an engineered tissue when seeded with adipose derived stem cells (ADSCs) producing the key extracellular matrix (ECM) proteins [ 7].
Sconocchia et al. also described a CD56+/CD33+ myeloid cell population which is able to differentiate in vitro into cells with a dendritic cell morphology [ 13].
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