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Previous studies have shown that the sensory epithelium is postmitotic, but it harbors cells that can behave as progenitor cells in vitro, including the ability to form new hair cells.
These studies revealed that when mouse BMSCs are compressed using these scaffolds they increase expression of critical markers of tooth differentiation in vitro, including the key transcription factors Pax9 and Msx1.
In recent years, a variety of organoids have been cultured successfully in vitro, including the stomach, liver, kidney, lung, gut, brain, and retina (Clevers, 2016).
To date, this is the first report showing a partial reversion of the MAR phenotype acquired in vitro (including the high-level of fluoroquinolone resistance).
Functional properties of RPE cells are also observed in iPS-RPE in vitro, including the transport of fluid across cells, as indicated by the formation of blister-like domes in the monolayer[39].
HN suppresses the neuronal death caused by all AD-related insults so far tested in vitro including the key cytotoxic molecule in AD, amyloid beta (Abeta) 1-42 [6], [5], [6].
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Culture methods for the enhancement of isolated primary hepatocytes or HLCs in vitro include the addition of GFs, TFs, and cytokines, the activation of signaling pathways, the use of an optimized matrix, and co-culture with various nonparenchymal cells.
The potent sPLA2 inhibitors, which broadly inhibits sPLA2s in the group I/II/V/X branch at low nM potency in vitro, include the functionalized indole scaffolds, such as indoxam, methyl-indoxam and LY315920, from Eli Lilly and Shionogi [ 68].
Conventional methods that enable the measurement of intra- or extracellular pH in vitro include the use of pH electrodes (Roos and Boron, 1981) and the use of optical dyes (Li et al., 2006; Thomas et al., 1979).
Some novel therapeutic strategies tested in animal models and in vitro include the administration of m-Tor inhibitors, PPAR γ agonists, vitamin D receptor activators, and Cox-2 inhibitors.
Limitations with the study of biofilms in vitro include the omission of host factors present at sites of infection (e.g. plasma proteins), which can stimulate biofilm formation [ 7], the lack of standardization among laboratory methods for characterizing biofilm phenotypes in vitro, and the lack of clinically available in vivo assays.
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