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Both FLSs and MSCs are contained within the mononuclear cell fraction in vitro, from which they can be culture expanded as plastic-adherent fibroblast-like cells.
Until recently six predicted domains have already been tested experimentally in vitro, from which five exhibit the predicted targeting function 8 (Resch and Hiss in preparation).
Furthermore, we artificially induced acidosis in vitro from which we derived direct information about the effects of selective acidosis on myocardial contractility.
LST is an examination that examines the sensitivity of a suspected material by measuring the proliferation of T cells to the material in vitro, from which one can conclude a previous in vivo reaction due to sensitization [ 13].
Following enzymatic release from the synovium, MSCs and FLSs are both contained within the plastic-adherent mononuclear cell fraction in vitro, from which they can be culture-expanded as fibroblast-like cells.
Nevertheless, it is interesting to notice that CIS derived from HSC-GT in vivo CD3+ cells displayed a different distribution of this histone modification as compared to NOT CIS subset and to the CD34+ cells in vitro from which they derived.
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Structural alerts represent the in vitro assays from which they were derived.
Total RNA (800 ng) from each target organ was subjected to reverse transcription, second strand synthesis and 1-round amplification by in vitro transcription, from which 20 µg amino allyl aRNA was labeled with Alexa Fluor 647 reactive dyes (Invitrogen).
Ring-constraint of the initial series provided access to a variety of in vitro active chemotypes, from which the indazole was selected.
PGPMs were selected based on initial in vitro laboratory tests, from which promising candidates showed considerable tolerance to high levels of stabilized NH4 + and ability to solubilize insoluble Ca3(PO4)2 (Nkebiwe et al. 2014 unpublished).
Furthermore, direct comparison of these studies is hampered by the use of different MSC culture conditions in vitro, different tissues from which the MSCs are derived, and a variety of different administration schedules currently used in vivo.
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