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Vignjevic, D. et al. Formation of filopodia-like bundles in vitro from a dendritic network.
Yui, S. et al. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell.
Yui, S. et al. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5 stem cell.
Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX.
As the antigen recognizing moieties, either monovalent or dimeric (head-to-tail) versions of an IgA-specific affibody (ZIgA1) were used, previously selected in vitro from a protein library based on combinatorial engineering of a single staphylococcal protein A domain.
In order to minimize the risk for the transplanted patients, a similar strategy is used when applying ex vivo expanded HSC in clinical trials: one unmanipulated unit containing long-term repopulating HSCs is transplanted together with hematopoietic (stem) cells that were expanded in vitro from a second unit.
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First, both SUV420H1 and SUV420H2 are capable of catalyzing H4K20me3 and can also catalyze H4K20me3 in vitro from an unmethylated substrate [10], [26].
The EGFP-specific antisense riboprobe was transcribed in vitro from an ApaI-linearized 383.pME-EGFP plasmid (Tol2kit), using T7 RNA polymerase and a DIG labeling kit (Roche).
At day 0, all hamsters were challenged in the submucosa of the right cheek pouch with HER-2-positive HCPC I cells established in vitro from an 7,12-dimethylbenz[a]anthracene-induced oral carcinoma.
Detailed studies of serial subcultures in vitro from an ovarian tumour showed that large changes in chemosensitivity occur within about 2 passages, in vitro, so that sensitivity testing can only be carried out using fresh explants or their first subcultures if any possible correlation between in vitro cytotoxicity and in situ response is to be studied.
In human NF1-the Alu repeat element is blocked in vitro from forming a nucleosome by the presence of a bipartite T14A11 tract sequence [ 51].
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