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First, in vitro experiments were conducted.
Key in vitro experiments were reproduced by M.v.G., M.R. and C.E.-N.
All in vitro experiments were carried out in triplicate unless specified.
Statistical analysis for the in vitro experiments were performed using the unpaired Student's t-test.
All in vitro experiments were performed in three to eight replicates and were repeated at least thrice independently.
The culture media of iNKT cells were changed every 2 3 days, and all of the in vitro experiments were performed after at least 10 days of culture.
The results of these in vitro experiments were unambiguous: receptor-bound aggregates activated Atg1, but only when Atg11 was also included in the mixture.
In vitro experiments were performed in rat superior mesenteric artery (SMA).
In vitro experiments were conducted by applying solutions of DTZ to the donor compartment.
In vitro experiments were performed by culturing primary hippocampal neurons from mice on micropatterned electrodes.
In vitro experiments were performed in the rat superior mesenteric artery.
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