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Thus, the diffusion that controls barrier crossing during protein folding is nearly as fast in cells as in vitro, even though translational diffusion of phosphoglycerate kinase in the cell is slow compared to in vitro.
HBsAg and HBV DNA production was stable for at least 22 days postinfection and, for the first time, demonstrates that 0.05 GE/cell results in stable HBV infection in vitro, even though observable HBsAg secretion at an MOI of 0.05 GE/cell was under the limit of quantification.
Hairy cells are not readily susceptible to EBV infection in vitro, even though they are EBV receptor-positive B cells.
We showed that, similar to ScYop1p, PbYOP1 generated membrane tubules when purified and reconstituted in vitro, even though a conserved amphipathic helix next to the transmembrane domain is less important than in ScYop1p.
Human anti-SP-22 antibodies that bound to and were eluted from an SP-22 affinity column reacted with gp120 in RIA and immunoblot assays but did not neutralize HIV or inhibit HIV-induced syncytium formation in vitro, even though these antibodies comprised 70% of all anti-gp120 antibodies in the test serum.
C. gattii cells with extracellular fibrils were also significantly more resistant to killing by human polymorphonuclear neutrophils (PMN) in vitro even though these PMN produced elaborate neutrophil extracellular traps (NETs).
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It is also striking that this competitive advantage for A. tumefaciens during intraspecies interaction is only observed in planta but not during in vitro growth, even though both antibacterial activity and type VI secretion were readily detected in vitro.
Recently, autophagic oocyte death was observed by two groups when individual isolated oocytes but not intact ovaries were cultured in vitro [10],[11], even though, the apoptotic cell death was also found to be the major mechanism of oocyte death, and when newborn mouse ovaries were cultured in the absence of serum [12].
However, we did not observe a direct modulation of CsA PK by its PD in our in vitro system, even though CsA interactions with transporters are known [ 29].
However, some graft transmitting genes were only identified in the field grafts, but not in the in vitro grafts even though these genes had comparable expression levels and diagnostic SNPs in both grafts.
For example, this cell line could be used for in vivo applications such as nuclear transfer after in vitro manipulation, even though aneuploidy may hinder meiosis and germline transmission.
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