Sentence examples for vitro enrichment from inspiring English sources

In vitro enrichment of SOD1 with oxidized glutathione (GSSG) recapitulates the increased abundance of apo-SOD1 monomers and small oligomers; this effect was also observed for the A4V variant, which contains a relatively low amount of endogenously modified GS-SOD1.

As a result, we identified a new protocol that allows for the in vitro enrichment and expansion in large scale of T lymphocytes obtained from PBMCs, which were compared to those obtained with TILs.

However, platinum treatment does seem to select for MMR deficient cells since in vitro enrichment for MLH1 deficient colon cancer HCT116 cells in a mixed cell population was seen after cisplatin treatment [ 53].

The percentage of CD62L+ or CCR7+ cells in the CD25+CD4+ population is higher in cultures in the presence of anti-CD4 (Fig. 4B) and the anti-CD4 treatment results in functional changes of CD62L CD25intCD4+cells, which again demonstrates the important role of this antibody for the in vitro enrichment and selection of Treg.

CMC cultures, analysed after in vitro enrichment in stem-like cells, display CD44 expression in a high number of cells, while, in the tumor of origin, CD44+ cells are confined in randomly spotted tissue areas, as described for human samples [ 71], explaining, as previously discussed, the apparent high percentage of CD44 negative tumors.

Our mass spectrometric elucidation of the SLP65 interactome circumvented common disadvantages associated with conventional approaches to characterize protein complexes, for example, weak signal-to-noise ratios, loss of binding partners owing to antibody-mediated complex disruption and in vitro enrichment of false positive candidates.

Despite the high-affinity, cooperative binding to multiple binding sites in vitro, no enrichment of this genomic region has been detected in the ChIP-chip assay.

Since AKAP13 and AKAP95 signaling cascades were significantly enriched in the ERαS305P positive patient subgroup, we tested in vitro whether this enrichment also implies a causal role for either of these two proteins in PKA-mediated ERα Serine 305 phosphorylation.

The step-wise neuronal differentiation protocols with all kinds of variations have been utilized by many laboratories to generate human neurons in vitro with high enrichment (Fig. 1A C).

Here, we report the in vitro differentiation and enrichment of keratinocytes from murine ES cells seeded on extracellular matrix (ECM) in the presence of Bone Morphogenic Protein-4 (BMP-4) or ascorbate.

After 4 rounds of in vitro selection and enrichment, the number of eluted phages increased 4000 fold (from 5.8×104 pfu to 2.4×108 pfu) compared to that of the phages in the first round (Table 1).