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With this in mind, we sought to address this question by introducing an in vitro embryonic stem cell (ESC) culture system, which successfully reduced the complexity of this organogenetic process down to the internal control of epithelial morphogenesis.
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Endocardial and myocardial lineages are segregated early during embryogenesis and such a cell fate decision can be recapitulated in vitro by embryonic stem cells (ESC).
Moreover, we found that HSCs from in vitro differentiated embryonic stem cells closely resemble definitive HSCs, yet lack a Notch-signaling signature, likely accounting for their defective lymphopoiesis.
We attempted to dissect early developmental stages of HSCs using an in vitro mouse embryonic stem cell (ESC) differentiation system combined with inducible HOXB4 expression.
Sasai et al. successfully created a complete, three-dimensional retina in vitro using embryonic stem cells [ 5].
Oct4 is an essential regulator of pluripotency in vivo and in vitro in embryonic stem cells, as well as a key mediator of the reprogramming of somatic cells into induced pluripotent stem cells.
Returning to biology to further validate the bioinformatic studies, we of course used the literature, but most importantly, we also tested our primary new hypothesis in vitro by embryonic stem cells (ESC) differentiation and in vivo during brain development.
These include proof of principle for restoring vision by rod-photoreceptor transplantation in mice with congenital stationary night blindness and advances in stem cell biology, which have led to the generation of complete optic structures in vitro from embryonic stem cells.
Recent studies of human astrocytes differentiated in vitro from embryonic stem cells reveal that those induced to a 'caudalized' phenotype through exposure to retinoic acid express higher levels of GFAP than those following the default rostral pathway of differentiation (Krencik et al., 2011).
At least some differentiation processes can be effectively mimicked in vitro using pluripotent embryonic stem (ES) and embryonal carcinoma (EC) cells, which have their physiological in vivo counterparts in cells of pre-implantation embryos (Andrews et al. 2001; Smith 2001).
The in vitro murine neural embryonic stem cell test (ESTn) was designed as an alternative for neurodevelopmental toxicity testing.
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