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Free pp60c-src was phosphorylated in vitro at a single site, tyrosine 416.
Photoactivation in vitro, at a clinically relevant light fluence rate (200 W/m2), demonstrated that tDodSNO released much higher levels of NO than either GSNO or SNAP.
Analyzing the cell lysates with CE in vitro at a series of time points gave a clear description of activation process of caspase-3 in cell apoptosis.
Here, we have prospectively identified a population of hESC-derived ROR2/CD13/KDR/PDGFRα cells that give rise to cardiomyocytes, endothelial cells, and vascular smooth muscle cells in vitro at a clonal level.
The objective of this study was to analyze the action of sildenafil citrate on normal human erythrocytes in vitro at a concentration (2.5 mg/mL) higher than the prescribed for clinical conditions.
After the SPRM cells were incubated in vitro at a concentration of 1×106/ml with both ATP (2 mM) and low dose 4-HC (2 μg/ml) for 3 h, 5×104 of the cells were then injected into lethally irradiated 9 weeks male BDF1 mice.
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To investigate the relevance of the kinetic analysis of isolated ATs in the context of intact PKSs, we complemented an in vitro AT-null DEBS assembly line with either trans-AT.
Bone marrow cells from DENV-2 infected mice were infected with different strains of DENV in vitro at an m.o.i. of 2 for 48 hrs.
Probucol significantly increased CETP activity in vitro at an even lower concentration of 1 pM up to 31.5% (p = 0.040).
In addition to ascertaining the likely safety of plant extracts for their potential use, standard cell-based toxicity assays are also performed in vitro at an early stage of the drug development process in order to remove high-risk materials [ 28].
The cells were maintained in vitro at 37°C in a humidified atmosphere of 5% CO2.
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