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To this end, we s.c. injected AlexaFluor-labelled BSA into mice and examined fluid retention/drainage using whole-body IVIS (Intra Vital Imaging System) imaging.
All morphological studies were performed by vital imaging using Plan-Apochromat 63x/1.4 oil immersion objectives.
Accordingly, we probed for microglial cell motion associated with SD using vital imaging of microglia in mature rat hippocampal slice cultures.
To ascertain whether low-dose IR was able to enhance angiogenesis in vivo, we used the transgenic zebrafish fli1∶EGFP, which allow intra vital imaging of the vasculature through fli1 gene promoter-driven expression of GFP in endothelial cells.
For vital imaging, zebrafish larvae at 6 dpf were anesthetized with a 610 µM solution of the anaesthetic 3-aminobenzoic acid ethyl ester (MS-222).
Studies on muscle fibre laser ablation and vital imaging proved that nerve terminal and Schwann cells remain in contact with the synaptic basal lamina.
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To obtain further insights into drug vector distribution changes in vasculature during tumor progression, we combined intra-vital imaging of metastatic tumors in mice, microfluidics-based artificial tumor capillary models, and Computational Fluid Dynamics (CFD) modeling.
Most have already had huge impact in basic cell and developmental biology, and applications in drug screening and intra-vital imaging are now being realised.
In vivo expression of split YFP protein fragments in liver hepatocytes and intra-vital imaging of subcutaneous tumor showed elevated YFP fluorescence upon apoptosis induction.
Intra-vital imaging of the tumors using a confocal microscope showed a significant increase in YFP-positive cells 22 and 32 h after CP injection, relative to that in saline-injected animals.
To extend these observations in ex vivo live imaging of infected tissue slices and intra-vital imaging are future challenges and will certainly reveal additional aspects of amastigote egress and reinfection.
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