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After the surface sowing on plate for spatulation and opportune incubation, we proceeded to enumeration of the positive cultures for the species searched and count of the number of colonies which developed for single plate (vital count, count in plate or count of the colonies), which is correlated to the explored anatomic zone numerousness.
Two count methods can be distinguished: -direct method (it uses the corpuscles count chamber and it counts both alive cells and dead cells); -indirect method or of the vital count or count in plate or count of the colonies, sow and subsequent counting of the developed colonies (sensible method).
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After vital counting, the suspension had a density of 2,5 × 106 vital cells/200 μl suspension (after centrifugation and re-suspension in PBS) before being injected into the animals.
After vital counting, the suspension had a density of 2,5 × 106 vital cells/200 μl suspension before being injected into the animals.
Many cells were located in the interface region of the enzyme-treated group (Fig. 2a,2b), but despite the apparently normal average vital cell count in the 150-μm broad band in the untreated control samples, the tissue in the interface region was almost acellular (Fig. 2c,2d).
Only cells with sufficient GFP and bisbenzimide signal as well as vital appearance were counted, aiming to count 100 cells per slide.
The raw data consist of birth and death counts from vital statistics plus population counts from official population estimates.
real estate mogul At last count, Vital estimates that he has 10 houses, each one radically different from the next -- though his notion of housing includes places like his latest one-bedroom, a hole on the side of Mount Tiggedit in Niger.
Vital cells were counted by means of trypan blue dye exclusion staining and adjusted to 5×106 cells/mL in fluorescence-activated cell sorter (FACS) buffer (PBS, 1% FCS, 0.1% sodium azide, 5% normal mouse serum).
Degenerated and vital neurons were counted from at least three independent hippocampal cell cultures.
SRT2379 also had no impact on vital signs, leukocyte counts, or coagulation activation markers compared with placebo.
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