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Cells were visualized with a microscope equipped with fluorescence filter.
To further confirm the apoptotic changes in P815 and BSR cells, Anexin V biotin-streptavidin FITcellsined cells were visualized with a microscope equipped with fluorescence filter (OLYMPUS OM52).
Stained cells were visualized with a microscope (IX81, Olympus, Tokyo, Japan) equipped with a CCD camera (Luca, Andor Technology, Tokyo, Japan).
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TMR and DAPI were visualized with a confocal microscope (Zeiss 510 META confocal microscope, Carl Zeiss Micro Imaging, Inc., Thornwood, NY) at 543 nm and 455 nm, respectively.
Immunostaining was visualized with a fluorescent microscope (Leica DM microscop, Bensheim, Germany).
Slides were mounted with Mounting Medium containing DAPI and PLA signal was visualized with a fluorescent microscope (Zeiss Axio observer).
The nuclear DNA was stained with SYBR green dye for 10 min and visualized with a fluorescent microscope (Zeiss Axio observer).
Finally, the cover slip was mounted onto a glass slide with 87% glycerol, and the immunofluorescence staining was visualized with a confocal microscope (Nikon, A1R).
The fluorescence signal from sfGFP and mCherry in these cultures was visualized with a fluorescence microscope (Nikon, Tokyo, Japan) equipped with B2 (excitation filter, 450 490 nm; barrier filter, 520 nm) and G2A (excitation filter, 510 560 nm; barrier filter, 590 nm) filters.
Fluorescence images were visualized with a fluorescence microscope.
The bound FITC-NLX was visualized with a fluorescence microscope.
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