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The partial release of lipids and mRNA out of the microparticle system, their accumulation in cells and the production of encoded protein were visualized via fluorescence microscopy.
The proteins were detected using horseradish peroxidase-conjugated secondary antibodies and visualized via chemiluminescence.
PCR products were visualized via electrophoresis on 1.5% agarose gels at 100 v for 1.5 hours.
After amplification, the PCR products were resolved on 1.5% agarose gel and visualized via ethidium bromide staining.
The protein was visualized via Coomassie stained SDS-PAGE and a Western blot with an anti-His antibody (Qiagen).
PCR products were visualized via 1% agarose gel-electrophoresis.
After standard pre-hybridization, hybridization, and washing procedures, bands were visualized via autoradiography.
Specific bands were visualized via the ECL kit according to the manufacturer's instructions (Amersham).
Gels were vacuum-dried onto filter paper and visualized via PhosphorImager (Molecular Dynamics, Sunnyvale, CA).
For large sets, smoothed histograms were generated and visualized via kernel density estimates computed by the "density" function.
PCR products were electrophoresed on 2% agarose gels (Seakem LE, Lonza Rockland, Rockland, ME) and visualized via ethidium bromide staining.
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