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Marco Gallio, who led the study, explained: "Our results show we can detect a specific pattern of activity between neurons in the brain, recording instantaneous exchanges between them as persistent signals that can later be visualized under a microscope".
Cells that migrated to the bottom of the membrane were stained with the cell stain provided in the assay kit for 20 min and visualized under a microscope.
Pollens were carefully squashed in the dye solution as evenly as possible, and pollen tube was visualized under a microscope at 100× magnification and about 200 pollen grains of each replication were observed.
After two weeks, the number of colonies that developed within each well was counted and visualized under a microscope after staining with 0.05% crystal violet for 1 hour and representative fields were photographed.
Colonies were visualized under a microscope and photographed.
The preparations obtained were visualized under a microscope (Eclipse E200-LED, Nikon, Tokyo, Japan).
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The samples were visualized under a fluorescent microscope (Nikon) or a Zeiss LSM 510 Meta confocal laser scanning microscope and analyzed by LSM 510 Image software (CLSM, Carl Zeiss).
The stained samples were first visualized under a light microscope, after which positively stained biofilms were examined under a transmission electron microscope.
Finally, the cells were washed three times with PBS and visualized under a fluorescent microscope.
Then, cells washed twice with PBS buffer solution and visualized under a fluorescence microscope [26].
Finally, the cells were visualized under a fluorescence microscope in 10 randomly selected fields for each group.
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