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The slices were also stained with Prussian blue and nuclear fast red and visualized under a light microscope (Olympus IX71).
The haemotoxylin and eosin (H and E) stained sections were visualized under a light microscope (Nikon eclipse 50i, Japan) under 20X.
Afterward, the cells were stained with Prussian blue and visualized under a light microscope (Olympus IX71, Olympus, Shanghai, China); the labeled MSCs were also stained with 1 mM Hoechst 33258 in PBS (pH 7.4) for 5 min, and fluorescent signal of MSCs was observed by a laser confocal scanning microscope with excitation wavelength of 488 nm (Leica TCS SP5).
The culture was propagated at 60°C until the spheroplast morphology could be visualized under a light microscopy (Fig. S3).
The stained samples were first visualized under a light microscope, after which positively stained biofilms were examined under a transmission electron microscope.
For light microscopic studies serial sections of both infected and uninfected roots of JG62 and WR315 were done every 24 h after inoculation, stained with Trypan blue and Lactophenol (Himedia Laboratories, http://www.himedialabs.com) and visualized under a light microscope.
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Leaf chlorophylls were removed with 95% ethanol before visualizing under a light microscope as described by Ishiga et al. [ 53].
PCR products were separated by electrophoresis on a 2% agarose gel supplemented with ethidium bromide and were visualized under a UV light.
The PCR products were visualized under a UV light transilluminator.
Signals were visualized under a standard light microscopy.
Thereafter, the cells were visualized under a transmission light microscope.
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