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While the significance of the proposed method is in enabling cryosurgeons to visualize the developing thermal field in real time, the broader significance of the current study is in its applicability to other energy modalities in medicine with very little modifications.
To examine the capability of DHM to visualize the developing tissue and cell morphologies of zebrafish embryo, the 40X MO is used to zoom into different regions of the developing zebrafish embryo (Figs. 6(g)–(i)).
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Using a technique called Light Sheet Fluorescence Microscopy, they visualized the developing embryo in 3D at 10x magnification.
injection of 0.05 ml 5% fluorescein isothiocyanate (FITC -labeled dextran 150,000 (Sigma-Aldrich, TauFITC -labeledmany) into the retro-orbital plexus, it was further possible to visualize the newly dextraning microvessels within the grafts and the surrounding host microvasculature by intravascular staining of the plasma.
Also, emanating from the border between the SVZa/RMS and the frontal cortex, chains of neurons (from ∼5 to ∼10 cells) were visualized entering the developing white matter towards the deep layers of the cerebral cortex (Fig. 13A A″ insets).
Applying selective plane illumination-based ultramicroscopy to entire wholemount-immunostained mouse embryos, we visualized the complete developing vascular system with cellular resolution.
Collins et al. (2014) therefore used immunofluorescence microscopy to specifically visualize the protein in developing oocytes.
In this work, liquid phosphoric acid was injected into polymer electrolyte membrane fuel cell (PEMFC) gas diffusion layers (GDLs) to visualize the invasion patterns developed at breakthrough.
To visualize the workspace, we develop a user interface to generate the cross-section of the reach envelope with a plane.
Transmission electron microscopy observations for specimens rapidly frozen below the glass transition temperature at particular times in the OOT processes are also conducted to visualize the transient structures developed during the OOT.
Recently, computer aided 3D reconstructions have been employed to visualize the morphological aspects of developing structures.
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