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KLS advised JIP and VR and helped visualize T opt and IL-tolerance.
To test whether loss of pFN alters fibrous cap formation, we immunostained cross-sections of similarly sized atherosclerotic plaques from ApoE−/−FNfl/fl and ApoE−/−FNMxCre mice for Mac-1 to visualize monocyte/macrophages, Thy-1.2/CD3 to visualize T cells, alpha-smooth muscle actin (αSMA) to visualize vSMCs and the ECM proteins FN and collagen type I (Col-I).
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WGA was used to visualize t-tubules of ventricular myocytes since it binds to sialic acid residues of the cell membrane glycocalyx [29].
Our animation program "LAVA" visualizes T-cell differentiation as cells moving across a landscape of hills and valleys, leading to attractor basins representing stable or semistable differentiation states.
T cells resuspended in imaging buffer and added to the flow cells 35 min after the final rinse and imaged immediately for 30 60 min. To visualize TCR, T cells were incubated in a solution of 1 μl Alexa 647 (Invitrogen Inc .-labeled H57 antInc .-labeled 100 μl imaging buffer for 20 min at 4°C prior to tH57regular imaging buffer resuspension.
Tang, Q. et al. Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice.
The sections were left unstained (to visualize red fluorescent T cells) or were immunostained for T cells with a green fluorescent mAb against CD3 or CD4.
Cell types, cell type-specific signature genes, dynamic gene expression patterns and enriched biological processes are visualized in t-distributed stochastic neighbor embedding (tSNE) plots, heatmaps, and profile charts.
A general linear analysis was conducted and the results were visualized as t-maps on the anatomical image.
Utilizing siRNA knockdown of tankyrase 1 in a variety of human cell lines, we found that its deficiency results in increased levels of telomeric recombination, visualized as T-SCE, in telomerase negative backgrounds.
RML 369-C [78] better resemble the flexible and tube-like Gram-negative bacterial conjugative pili, which are quite larger and easier to visualize than the T pilus and related structures [101].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com