We plotted the occurrences of the three species in this same space, to visualize the section of the climatic space occupied by each species.
The 3D reconstructions obtained by micro-tomography are used to visualize cross sections of the droplets in any spatial directions.
was used to visualize stained sections.
To visualize cross-sections of mouse skin, whole skin tissue was embedded in O.C.T. compound (Tissue-Tek), and 10 µm skin sections were cut on a Leica Cryostat.
To visualize neurons, sections were incubated in donkey anti-mouse Alexa Fluor 488 (Jackson Laboratories) to visualize NeuN-positive cells.
To visualize pericytes, sections were incubated in bovine anti-goat Cy3 (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) diluted 1 100 to detect PDGFRβ.
To assess the collagen matrix, we performed picrosirius red staining and visualized the sections by polarization microscopy (Olympus BX51, Olympus, USA) as previously described [22].
Hypoxia tumor area was determined by visualizing stained sections and quantifying the number of stained/unstained cells in 5 high power fields (x400 magnification).
Visualizing single sections of interest therefore saves time.
We visualized the sections under a four-color fluorescence microscope, BIOREVO (Keyence Corporation, Osaka, Japan).
We visualized the sections using a Nikon A1 laser-scanning confocal microscope.
