Preparation of extracts from mouse tissues or whole cell dishes, electrophoresis, blotting, visualization and stripping of membranes were performed as described [84].
Our application proposes to enrich inspections by superimposing information on the device screen with the same point of view of the camera, providing an intuitive visualization of buffer strip location.
To evaluate the performance of our segmentation and tracking algorithms, we used a film strip visualization with computationally annotated cell contours and manually examined images for 2340 single cells transfected with EF1α-VC5 over the 24 hour time course.
After visualization, blots were stripped and incubated with anti-β-actin antibody (Abcam, Cambridge, MA) at 1 10,000 in blocking buffer, then exposed to secondary antibody as above.
Following visualization, the blots were stripped by incubation in stripping buffer (Restore; Pierce Biotechnology, Inc., Rockford, IL, USA) for 15 min and then incubated with antibodies of rabbit anti-NR2B (1 : 1000; Millipore) or rabbit anti- β-actin (1 : 1000; Abcam).
After visualization, the membranes were stripped (30 min, 50°C) in 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS and 50 mM 1,4-dithio-L-threitol, blocked, and reprobed with anti-pan-Erk1/2 antibody (Cell Signaling).
Hybridization signal intensity of each virus-specific probe element from the microarray is converted to color visualization scheme and depicted as a vertical strip.
Fig. 1 New imprint method for the visualization of microbial contaminations on processed meat strips.
A retractor was placed into the wound under direct visualization, and cortical and cancellous bone graft strips were harvested.
The inability of the CASP1-GFP ring to retract from the Casparian strip upon plasmolysis allow for easy visualization of this phenomenon.
