Then, tissue sections were incubated with rabbit polyclonal anti-ISG15 antibody (30 μg ml−1) and visualised using the Envision™ visualisation system.
The primary antibodies were visualised with the Envision™ visualisation system and the chromogen DAB+ from DakoCytomation A/S (K3868), followed by counterstaining with Mayer's haematoxylin and mounting in Aquatex® (1.08562; Merck, Darmstadt, Germany).
Then the slides were washed for 10 min with PBS and were visualised with the EnVision system using diaminobezidine tetrahydrochloride as a chromogen.
Diagnostic PET and SPECT imaging are routinely used to visualise dopaminergic system integrity, using tracers which target either dopamine synthesis and storage capacity (l-3,4-dihydroxy-6-[18F]fluorophenylalanine = [18F]FDOPA) or dopamine transporter density (e.g. [123I]FP-CIT; [2]).
The intuitive DEA score can help service managers and policy makers visualise the system performance and examine the potential impact of ineffective resource use [ 20].
This study primarily provides a means to visualise respiratory system elastance continuously, thus allowing PEEP to be titrated to minimal elastance [ 12, 14, 15], and it was suggested that it can be done using either incremental or decremental phase of a staircase RM.
Visualising complex interacting systems.
In the last decade, the zebrafish, owing to the transparency of its embryo and the ease with which it can be genetically manipulated, has emerged as an exquisite system for visualising the immune response to pathogen infections in vivo and in real time.
Finally, excretory phase imaging obtained at least 5 20 min later allows visualising the opacified collecting systems and detecting iodinated urine leaks and urinomas.
The graphics quickly evolve and begin to form geometric shapes that bubble and dance as the line of shapes shifts forward, growing and twirling, like an audio eco-system, lucidly visualising the alternating rhythms of the track.
