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Firstly, they can be externally detected in tissue, preincision, secondly, they are of similar dimensions to the radiotracer colloid, and thirdly, their brown-black appearance acts as a visual stain.
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This resulted in a subset of 123 images that could be used to compare how the predicted localizations related to the visual staining patterns.
Independently, we also calculated the accuracy of cytoplasmic (or plasma) membrane localization predictions in relation to the observed, visual staining patterns.
According to these studies, the clone MIB-1 appears to have a higher sensitivity for detecting the Ki-67 antigen than other antibodies and it also gives the best visual staining.
We considered visually-scored staining intensity values both alone, and in combination with staining extent as calculated histoscores, but found that the latter combination did not produce additional insights beyond those obtained through comparing visual staining intensities alone (Tables 2, 3).
For better visual observation, staining with Coomassie Brilliant Blue R-250 was carried out on detached leaves using the procedure described in Riaz et al. Six plants of each accession were propagated from green cuttings and established in a greenhouse.
By visual inspection, the staining patterns of these probes appeared similar to the organelle-associated staining pattern of the reference probe, although there was substantial variation in the overall intensity (Figure 6).
The most common method of quantifying immunohistochemical staining patterns is by visual estimation of marker stain intensity and area.
By visual inspection, the staining patterns of the probes revealed roughly similar numbers of membrane- and organelle- staining probes, and a smaller fraction of nuclear-staining probes.
Only compounds with Tc < 0.25 in the aldehyde building block (Figure 6) yielded clear visual differences in staining patterns as compared to the reference probe, explaining the absence of quantitative correlation between variations in Tc and variations in image features [27].
Test results were read after 10 min by visual inspection for staining of the antigen and control lines.
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