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User-defined visual scripts imitate the keyboard and mouse commands that an analyst would use to manually change parameters and launch an LC-MS/MS analysis, enabling MUSCLE to control multiple LC and MS parameters on any instrument (Section 2.1).
All questionnaires were administered in private interview rooms via the Questionnaire Design System (QDS), a computer-administered self-report interview system that includes audio and visual scripts (i.e., participants are able to hear and read questions).
Once visual scripts are set up to control a particular instrument, an experiment configuration can be created, which contains all the information MUSCLE requires for an automated optimization study.
These visual scripts can be saved and later reused or modified for reuse on different analytical instruments and can be shared between laboratories using an import and export function.
Here we use the Sikuli Java library (Yeh et al., 2009), providing a powerful and flexible API to allow users to create visual scripts that can: click/double click on selected objects on the screen, enter text into text fields and press selected keyboard keys, e.g. Enter.
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