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Developed visual assays were performed in two steps of hybridization and detection.
These features have facilitated the development of visual assays with high spatiotemporal resolution to monitor specific subcellular processes.
Bioinformatics, biochemical and visual assays were employed to investigate the identified gene targets and to reveal their involvement in i-Extract-induced cancer cell killing.
Other than visual assays, changes in gene expression can be investigated at either the whole transcriptome level or in more focused subsets of genes (Hegedűs et al., 2009; Rotman et al., 2011; Kanwal et al., 2013; van der Vaart et al., 2013; Veneman et al., 2013).
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In this study, we developed a rapid visual assay for detecting BVDV-RNA using PNA and unmodified AuNPs.
The label-free colorimetric assay developed was estimated to have a 10.48 ng/reaction BVDV-RNA detection limit for the visual assay and 1.05 ng/reaction BVDV-RNA using a spectrophotometer.
In the present study, the color changes of AuNPs when facing a PNA-complementary RNA, and PNA/non-complementary RNA were used to develop a rapid, label-free visual assay for the detection and quantification of BVDV-RNA.
Using this caspase-activated fluorescent dye as a visual assay, we have now screened thousands of compounds.
RNAi against the mitotic kinesin, Pavarotti, was performed in parallel as a positive control as they fail to undergo cytokinesis and become multinucleated, providing a visual assay of knockdown effectiveness.
When re-tested in a qualitative visual assay of PED-6 metabolism, 15 of these compounds were considered active in a dose responsive fashion (0.3% of the total number screened).
This visual assay further confirms the intermembrane localization of IL-1β in the phagophore and autophagosome.
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