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In addition to this, offline results obtained with a particle vision microscope system are compared to verify the results obtained inline.
Numbers of fluorescence signals were counted independently by two investigators using an Axio Vision microscope (Carl Zeiss, Oberkochen, Germany).
Optical microscope investigations of the films were performed with a Zeiss Axio Vision microscope between crossed polarizers.
The number of fluorescence signals was counted independently by two investigators using an Axio Vision microscope (Carl Zeiss, Oberkochen, Germany).
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After further washing steps, the samples were mounted using a DAPI 4′,6-diamidino-2-phenylindole -containingg medium and imaged on a Delta-vision microscope system using either a fluorescein isothiocyanate or tetramethylrhodamine isothiocyanate filter.
All pictures used for counting procedures were taken in one session and all counting procedures were performed on light microscopic photographs taken using the ×20 objective on a Carl Zeiss Vision light microscope equipped with Carl Zeiss Imager.Z1 and with an AxioCam MRc5 from AxioLab.
Furthermore, a vision measuring microscope was used to investigate the quality of surfaces that impaired by the wear test.
After three washes, slides were mounted with ProLong Gold Antifade Reagent that includes 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and imaged using Delta vision Inverted microscope (Olympus).
The basic ideas are essentially included in Abbe's theory of vision in a microscope first published in 1873; the subsequent illustrative experiments of this theory, notably by Albert B. Porter in 1906, are certainly simple examples of optical processing.
These slides were studied under a binocular microscope (Vision V4000).
In order to define the cut-offs between high, low and absent ALDH1 expression, 50 randomly selected microscope vision fields were analyzed and a total of 1.500 cells presenting high, low or no ALDH1 expression (500 cells each) were measured by the ARIOL system.
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