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After 30 days, clearly visible shoot primordia with no visible signs of microbial presence were transferred to culture boxes with vented lids and fresh medium.
Prolongated culture on PCZ supplemented media led in many cases to the disappearance of a morphologically visible shoot meristem (data not shown).
If there was visible leaf induction observed in the tested concentration, the callus was assigned the number 2. When the callus turned green but did not produce a visible shoot, then it was given the number 1.
The extent of callus growth prior to visible shoot regeneration was also, on average, two months longer for P. trichocarpa Nisqually-1 compared to P. tremula x alba 717-1B4, and the frequency of shoot initiation per callus much less.
Thus, the number 2 signified that all of the calli tested at the specific combination of auxin and cytokinin concentrations produced visible shoots, whereas the number 0 represented a lack of visible shoot formation from calli.
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If conditions are right, the Taurids should produce about five visible shooting stars per hour, and the Leonids 15 or more, although the Taurids can be slower-moving and brighter.
We assigned the numbers 0, 1, and 2 to calli having no shoots, greening only, and visible shoots, respectively; thus, a number close to 2 meant that most of the calli tested at the specific combination of auxin and cytokinin produced visible shoots, whereas a number closer to 0 indicated that none of the calli formed visible shoots.
For each medium with the different auxin and cytokinin concentrations, calli were serially sub-cultured for 6 months, transferred and incubated for 4 weeks until visible shoots formed.
AS I dig the cannas, I divide off tuber pieces (making sure each division has a visible growing shoot), pot them separately and let them sprout indoors.
For the 8 day sample from SIM, only explants that had visible emerging shoots were chosen in an effort to ensure that transcriptional changes related to shoot regeneration could be detected.
But asked if the government's move would mean he would be less visible, Varoufakis shot back with a rhetorical: "The finance minister?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com