The phenolic compounds were identified and classified into three groups based on their UV-Vis spectra, retention times, and the qualitative results obtained from UPLC-PDA-ESI-MS (the accurate mass and the MS fragmentation patterns).
Based on the UV-VIS spectrum, retention time, and the comparison of MS spectrum obtained by Q-TOF LC/MS to fragmentation data from previous literature reports [ 35, 37], compound A was provisionary analyzed as a procyanidin B1.
Compounds C was tentatively identified as a procyanidin B2, based on the UV-VIS spectrum, retention time, and the comparison of MS spectrum obtained by Q-TOF LC/MS to fragmentation data from previous literature reports [ 35, 37].
Behavioral change resulting from knowledge gained was not directly measured; subsequent work might assess knowledge retention vis-à-vis behavior modification at later time points after program exposure.
A peak corresponding to POG-Tγ-VIS with a retention time of 24.05 min completely shifted to a new peak with shorter retention time of 23.75 min (trace c).
Individual polyphenols were identified by comparison and combination of their retention time, UV-Vis and mass spectra of the peaks with those of authentic samples or isolated compounds previously characterized [ 42].
The detected compounds were identified by comparing their retention times, UV-Vis spectra, and fragmentation patterns in MS spectra with those of the reference compounds and the literature data [ 19– 22].
GIT: gastrointestinal tract; CFU: colony forming units; DAD: diode array absorbance detector; ESI: electrospray ionization; HPLC: high-performance liquid-chromatography; MS: mass spectroscopy; UV-vis: ultraviolet-visible light; Rt: retention time The authors declare that they have no competing interests.
Main phenolics of the NAF from black soybean seed coat were qualitatively identified by the Q-TOF UPLC/MS analysis for retention time, UV-VIS spectrum, MS scan for mass fragmentation, and the comparison of MS spectrum obtained by Q-TOF LC/MS (MS/MS) to fragmentation data from previous literature reports [ 35– 35].
In the HPLC analysis, Tγ-VIS gave a peak with a retention time of 17.85 min (trace a).
This article presents data describing the molecular changes that occur to a TPx mimic upon exposure to H2O2, and then the thiol mercaptoethanol, as characterised by UV vis spectroscopy and HPLC retention time.
