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Samples were processed and viruses were identified using antigen detection [10].
Samples were cultured using primary monkey kidney cells, and influenza A and B viruses were identified using immunofluorescent staining with influenza-specific monoclonal antibodies.
The respiratory viruses were identified using conventional diagnostic methods, including direct immunofluorescence testing (DIFT) on respiratory samples (e.g. nasopharyngeal aspirates [NPAs]; broncho-alveolar lavages [BALs], tracheal aspirates [TAs], and oral swabs), with confirmation and typing by type-specific DIFT and viral culture.
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Antibodies specific to the influenza pandemic virus were identified using hemagglutination inhibition and microneutralization assays.
Systematic analysis of suspected cases [1] was undertaken and the virus was identified, using molecular methods, in the Public Hospital virology "Level A" laboratories of the seven French Defence Zones.
The H9N2 virus was identified using a standard HI assay.
The virus is identified using reagents provided by WHO Collaborating Centers.
No novel HoBi-like virus was identified using panpestivirus primers described by Letellier et al. [ 31] that are able to detect HoBi-like virus [ 37, 38]; moreover, in central and southern Italy, a protocol for atypical Pestivirus detection [ 14] has been also applied.
A neutralizing epitope, as well as other potential antigenic sites of a SAT2 foot-and-mouth disease virus (FMDV) were identified using phage-displayed scFvs.
Viruses were isolated and BAV isolates were identified using described procedures (8 ).
Quasispecies of infectious bursal disease virus (IBDV) vaccine and wild-type strains were identified using real-time RT-PCR at a region of the viral genome known for sequence variability.
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