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Another sequence-based molecular classification method for viruses is based on pairwise identities of virus sequences within a virus family.
Further subdivision of the RNA viruses is based on whether the RNA genome is segmented.
The Baltimore classification of viruses is based on the mechanism of mRNA production.
Most information on transmission of influenza viruses is based on older experimental studies, inference from observations during outbreaks, and studies with other objectives, especially the assessment of vaccine or drug efficacy.
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The part dedicated to influenza viruses was based on consensus sequences specific for one gene generated from global alignments of a large number of influenza virus sequences available in databanks.
The criterion used for future identification of the H1N1pdm viruses was based on the detection of BC signatures identical to those of A/California/04/2009 in at least five of the six targets in the "core" genes.
The procedures used for the collection of viruses were based on previous studies with some modifications.
Recognition of respiratory viruses was based on the detection of viral antigen in the nasopharyngeal sample or a 4-fold or greater increase in serologic titres.
Quantification of viruses was based on the quantitative PCR protocol described by Martenot et al. [ 29] and virus quantity was reported as genomic units (GU) of virus per nanogram of genomic DNA extracted [ 17, 33].
Three types of influenza viruses, namely, influenza A, B, and C type viruses, are based on the unique identity of the internal proteins, the nucleoprotein (NP), and the matrix (M1) protein.
The only gene that is conserved in all dsRNA viruses along with positive-strand RNA viruses is the RNA-dependent RNA polymerase (RdRp), and most of the conclusions on evolution of RNA viruses are based on RdRp phylogeny supplemented by phylogenies of other genes that are conserved in subsets of viruses and comparative analysis of genome organization [ 6].
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