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We believed that the analyzed viruses in this study were isolated from patients without oseltamivir-treatment, although we did not have their clinical record of drug therapy.
The nucleotide sequences of influenza viruses in this study have been submitted to GenBank and their accession numbers are HQ291845-HQ292044.
It is striking that N66S in PB1-F2 is associated with increased virulence in a mouse model [57] but viruses having S at residue 66 of PB1-F2 arareand and were not present in the H9N2 viruses in this study.
Phylogenetic analysis of all gene segments of the A/H5N1 viruses in this study did not allow us to pinpoint the origins of the different viruses and it remains unclear how the three distinct A/H5N1 sublineages entered the continent and spread so rapidly.
RSV and HAdV were the most commonly detected viruses in this study.
The viruses in this study were most closely related to those from the Indian subcontinent.
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They scoured published genomes from all groups of organisms-although they didn't include viruses in this study-focusing on pieces of proteins known as domains.
HSV-1 strain KOS was used as the parent wild-type virus in this study.
The use of MS2, a nonenveloped virus, in this study does not accurately reflect the potential efficacy of the steam bag against enveloped viruses including 2009 H1N1.
We were not able to perform culture for influenza virus in this study, so it is possible that some of the infected travellers were not shedding viable virus.
Influenza illness observed in the ferrets infected with H5N1 virus in this study closely resembles influenza in humans with H5N1 infection, who present with fever, cough, shortness of breath, and radiological evidence of pneumonia [23].
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