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These results demonstrated that N protein contains non-essential regions for virus viability in cell culture.
With Dr. Shaman, she is currently working on a project studying influenza and other cold virus viability in NYC subways, as well as a health assessment on indoor temperature and humidity conditions in NYC apartments.
These results support the Zust model for the coronavirus 3′UTR and suggest that the S3 stem is required for virus viability.
It is also unclear whether virus internalization by higher trophic-level organisms has a protective or a detrimental effect on virus viability and transport in pond systems.
It was confirmed that this loss was due to sorption rather than inactivation by using lysis-based recovery techniques and qPCR measurements that do not depend on virus viability.
Prior studies on PRRSV strain VR-2332 non-structural protein 2 (nsp2) had shown that as much as 403 amino acids could be removed from the hypervariable region without losing virus viability in vitro.
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Fourthly, we did not assess whether the detected virus particles could replicate in human cells and therefore had no information regarding the viruses' viability or infectivity, both of which are clinically important.
However, previous studies have shown that mutations that inactivate alternative splice sites in the M gene decrease the viruses viability and growth rate.
In laboratory experiments collecting aerosolized influenza virus, the viability of virus collected with an SKC BioSampler was about 4 times higher than the viability of virus collected with the NIOSH sampler [17].
After 24 h incubation with the virus, cell viability was determined via crystal violet staining (570 nm absorbance).
10 candidate positions in the RdRP nucleotide sequence from FLIC DENV-2 were selected, 37 codon substitutions proposed, and predictions of resulting changes in the virus growth (viability of the mutant) made.
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