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The swabs were placed in 1 mL of virus transportation medium (Hanks balanced salt solution) as described in Wallensten et al. [7] and kept on ice until they were stored at −80°C.
Nasopharyngeal swabs preserved in virus transportation medium were sent to the World Health Organization (WHO) associated National Influenza Centers of Valladolid, Majadahonda and Barcelona, Spain for viral diagnosis purposes.
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Although the use of viral transport medium should ensure that virus will maintain viability under refrigeration, we pre-tested the effect of transportation procedures on virus viability by using a sample of viable human influenza virus in virus transport medium (provided by ProvLab) in 2 pre-tests (including courier to Memphis and culture in Memphis).
Even when using PRRSV-negative suppliers, pigs may come into contact with the virus during transportation [ 5].
The bad news is that because of modern transportation, the virus could spread around the globe within days, and before any symptoms had appeared.
Identification of the factor(s) to link the K+ transportation and virus movement will help elucidate the mechanism of how the GmAKT2 overexpression in soybean results in greater resistance to SMV.
It is already reported that viruses hijack the plant RNA transportation system for disease spreading.
We did not test these fluids from the common transportation truck for influenza viruses; this is also a limitation of the study.
These carriers could have transmitted the viruses to the sampled horses during transportation, training periods, race competitions, or breeding [ 2, 10].
The elaborate transportation system for a flu virus adds to the chance nature of its evolution.
Above all, we have the free treatment for the virus and we do not suffer the transportation problem going to [nearby town].
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