Exact(1)
The viral vectors were stored at -80°C in virus preservation solution (10% glycerol, 0.585% NaCl, 0.02% MgCl2·H2O, 0.01 M Tris-HCl, pH 8.4) at a concentration of 1 × 1012 VP/ml.
Similar(7)
We chose 30 minutes based on time course studies that showed that 30 minutes allowed for inactivation of the virus while maintaining preservation of the pp65 antigen (data not shown).
Although many poxviral proteins retain sequence homology and functional similarity with host proteins, it has recently been shown in the case of Vaccinia virus protein N1 that preservation of structure and function can occur in the absence of significant sequence similarity [52].
Studies on viral transport media for the preservation of virus viability have concluded that the best transport media for specimens at risk of being delayed by long transit times and exposed to significant temperature variations en route, are those that contain 2% FBS (i.e., CVM-Copan Diagnostics, Corana, CA, and M4-Multi-Microbe, Micro Test, Inc., Snellville, GA).
This technology has been used to support the dry state preservation of viruses, eggs, sperm, and macrophages.
We have been applying this technology to the preservation of viruses, sperm and eggs, as well as macrophages, a representative nucleated mammalian cell line.
Although experimental studies have not yielded a well-defined period at which viable variola virus can survive in a preserved state (either deliberate experimental preservation or part of the natural process of tissue preservation), there is an overriding conjecture reached by these studies.
When the reservation lines go silent, owners will find that the company stops seeming like a bunch of charming sticklers and starts seeming like a virus that cares about self-preservation more than the well-being of its host.
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