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Sabin poliovirus type 1 from the original stock (passage 0) was compared with the passaged virus (passage 5).
The engineered receptor tropisms were stably maintained during multiple serial virus passage without reversion to native receptor usage, even on cells offering the choice of both native and alternative receptors.
Using a combination of targeted mutagenesis followed by serial virus passage in ferrets, we investigated whether A/H5N1 virus can acquire mutations that would increase the risk of mammalian transmission (34).
"The time to generation of a virus by de novo engineering from raw sequence data is probably longer than the age-old method of virus passage in mammals/mammalian cells".
In the derived progeny, the vector elements were only traceable until virus passage two (Fig. 3B).
inoculation of two 5-day old chickens per lineage with 100 ul of virus passage as previously described [21].
Similar(36)
In experiment 4 (see supplementary materials), we investigated whether airborne-transmissible viruses were present in the heterogeneous virus population generated during virus passaging in ferrets (fig. S4).
Because all poliovirus populations are heterogeneous [18], [19], we used virus passaged in the absence of drug as a reference.
The number of virus passages in eggs was limited to one in order to restrict genome modifications linked to laboratory manipulation.
Numbers following passage source represent the number of virus passages.
This suggests that the virus passaged in the KG1 cells had an enhanced replication speed.
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