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Virus packaging was performed in HEK 293T cells after co-transfection of pWPXL-638 or pWPXL-PLD1 with the packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 2000 (Invitrogen).

Considering the impact factors that lentiviral vector systems can deliver exogenous DNA fragments of approximately 8 kb, viral titers are inversely proportional to the size of inserted fragments and virus packaging efficiency, a maximum size of the 5′-regulatory region of the CMV gene that can be packaged into virus particles was selected.

The Emuc-TVA construct was firstly cloned into a donor vector (pAdT) containing an EGFP expression cassette as a reporter; then the adenoviral vector expressing the Emuc-TVA and EGFP (named ADV-Emuc-TVA) was generated by homologous recombination and virus packaging (Fig. 1A and Supplementary Materials and Methods).

Among the candidates, the two frontrunners are TKM-Ebola, an RNA inhibitor of the virus packaged in a lipid nanoparticle, and ZMapp, a laboratory-made cocktail of three Ebola antibodies that shot to fame in August when it was given to Brantly.

Virus packaging was achieved with ViraPower™ plasmid mix (Invitrogen).

Phoenix ecotropic virus packaging cells in 10 cm-culture dishes were transfected by phosphate calcium precipitation with 10 µg of retroviral vector.

For virus packaging, HEK 293T cells were transfected with pDSL-hpUGIH plasmid containing shRNA expression cassette by using the three-plasmid transient transfection system [47].

We thank N. Kurebayashi for valuable discussions, and Y. Wang and R. Hirsh for helping with HSV virus packaging and 1B5 cell culture.

To prepare HIV pseudotype virus packaged by vesicular stomatitis virus (VSV) Env, an HIV proviral construct encoding a luciferase reporter gene (pNL4-3.Luc.R−E−) was cotransfected with a VSV Env expression vector in 293T cells as described [36].

Virus packaging was performed in HEK 293T cells after the cotransfection of 20 µg pGCsil-GFP-let-7f vector with 15 µg of the packaging plasmid pHelper 1.0 Vector and 10 µg of the envelope plasmid pHelper 2.0 Vector using Lipofectamine 2000 (Invitrogen).

Virus packaging was performed in 293T cells.