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CpGV is sprayed frequently during a season, whereas soybean can tolerate some damage and the virus is applied fewer times per season.
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Three days after the virus was applied, 85% of the cells in the culture dishes were infected.
The infection was not detected in the dendritic cell rich squamous epithelium to which the virus was applied; instead, it was primarily in CD4+ tonsillar T cells, close to the specialized antigen-transporting epithelium of the tonsillar crypts.
In contrast, when the virus was applied to cultures of fetal kidney cells, embryonic stem cells, and undifferentiated iPS cells, it infected fewer than 10% of the cells by day 3. Immature neurons derived from the neural progenitor cells were also less susceptible to the virus; 3 days after receiving a dose of the virus, fewer than 20% of those cells were infected.
1 ml of virus was applied 30 min later.
The virus was applied to cells for 4 h in the absence of serum.
In short 150 pfu of virus are applied to each well of confluent MDCK cells in 6-well plate format.
One mL aliquots of radiolabeled virus were applied to the columns, and 10 drop fractions were collected using an automated fraction collector.
A 1.0-µL aliquot of intact virus was applied to the Ag nanorod array substrate and allowed to evaporate at room temperature prior to spectrum acquisition.
To confirm the quality and purity of the virus preparation, purified virus was applied to a formvar carbon coated grid for 1 min, and immediately stained with 1% uranyl acetate for 30 s [26].
In subsequent passages, 200 μl of neat virus from the previous passage (1/10 of total virus) were applied to a new monolayer of cells.
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