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Mosquitoes were counted, identified, frozen (-80°C) and sent to the National Arbovirus laboratory in Rome for virus examination.
The clinical signs, findings at necropsy and absence of other pathogens corresponded well with EMPF, and thus samples from lung tissue and tracheal wash were submitted for further virus examination.
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On the basis of pathologic examinations, virus isolation, virus identification and genetic characterization, we found an association with the new DTMUV.
An accurate examination of virus specific Ab subfamilies elicited during infections may help further characterize the complex interplay between viruses and the humoral immune response, and be useful in the design of future monoclonal antibody (mAb -based anti-infective prophylactic and therapeutic strategies.
Tissues were collected for pathological examination and virus isolation.
Treatment of viral assemblages with DNase I and examination of virus containing agarose beads using confocal microscopy was used to address these issues.
Virus labeling and histochemical examination were performed according to van Riel et al. (5 ).
Four months after initial examination, the virus has been cleared but the patient still has intermittent confusion and language defects.
Viral RNA was recovered from all tissues sampled at postmortem examination, and virus was reisolated from lung, brain, lymphoid tissues, and kidney (6 ).
Patients' clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells.
In addition to serology, it is therefore imperative that examination for virus or viral antigen is performed, e.g. by PCR, cell culture or in situ detection methods.
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